Fig. 4

Analysis of transmission scores for all ASVs. All hen ASVs (A) and chick ASVs not appearing in hens (B), binned by their transmission score. Gray bars in (A) represent hen ASVs that did not appear at all in chicks (transmission score = 0%). Data are presented as the mean ± SD, for the untreated group consisting of all three rounds; egg disinfection and antibiotics groups were present only in one round, thus their data presents only the mean. The number of ASVs in each bin in indicated above the bar. Each bin of the treated chicks was compared with its counterpart of the untreated chicks (n = 3) by two-tailed one-sample t-test; *p < 0.05, **p ≤ 0.01