Fig. 3

Analysis of hens’ and chicks’ shared and non-shared ASVs. A All untreated hen fecal samples were pooled and compared to the pooled chick samples of each specific day and round. Number of ASVs present only in hens' feces (gray bars), in hens’ feces and in chicks (light color bars) and only in chicks (dark color bars) are presented. B, C Percent of hens’ ASVs that also appear in chicks (B) and number of ASVs that appear only in chicks (C). Data are presented as the mean ± SD, for the untreated group consisting of all three rounds; egg disinfection and antibiotics groups were present only in one round, thus their data presents only the mean. Two-tailed one-sample t-test against data of all rounds (n = 3) of untreated chicks; *p < 0.05